Abstract

Introduction

Neurodegenerative and psychiatric disorders are often linked to oxidative stress and mitochondrial dysfunction, necessitating novel therapeutic strategies. Bacillus coagulans, a probiotic known for its role in gut-brain axis modulation, has been investigated for its potential neuroprotective and antioxidant properties. Assessing its effects on neuronal cell viability and oxidative stress may provide valuable insights into its therapeutic potential.

Objectives

This study aims to evaluate the cytotoxicity and oxidative stress modulation of Bacillus coagulans in SH-SY5Y neuroblastoma cells. Escitalopram was used as a standard to compare its potential neuroprotective and antioxidant effects.

Methods

Cytotoxicity studies on SH-SY5Y were aassessed by MTT Assay at 5 different concentration. Oxidative stress levels were analyzed using ROS (DCFDA) to evaluate antioxidant activity. Escitalopram was used as a reference standard for comparative analysis.

Results

The study provides insights into the effects of Bacillus coagulans on neuronal cell survival and oxidative stress modulation. Observed cytotoxicity and antioxidant activity suggest its potential neuroprotective role, supporting its relevance in neurological health.

Conclusion

The findings highlight the neuroprotective and antioxidant potential of Bacillus coagulans in SH-SY5Y cells. Its ability to regulate oxidative stress may contribute to novel therapeutic approaches for neurodegenerative and psychiatric disorders, warranting further investigation.